Cutting edge techniques for standardized services
TISSUE CLEARING IN BIG SPECIMENS
Tissue Clearing allows the TEFOR Paris-Saclay to image big samples in-toto and high resolution. Using a surfactant-based and non-toxic protocol enables us to run these experiments regularly and in big numbers.
HIGH REFRACTIVE INDEX MATCHING
In addition to the tissue clearing, the TEFOR Paris-Saclay developed a protocol for matching the naturally in-homogeneous refractive index of specimens. This facilitates high-resolution deep imaging.
CONFOCAL AND MULTIPHOTON MICROSCOPY
Our confocal and multi-photon microscopes are equipped with Leica’s new high refractive index immersion objectives. With 25x/1.00 IMM and a working distance of 6mm these setups allow imaging of thick samples.
DATA MANAGEMENT, ANALYSIS AND VISUALIZATION
High resolution data of big specimens quickly end up rather heavy. We developed a data treatment and management pipeline which allows us and the users of our service to easily access our results.
TPS IN THE MEDIA
On 18.4. Thierry Lhermitte, as the ambassador of the Fondation pour la Recherche Médicale, visted the group of Philippe Vernier at the CNRS Campus at Gif-sur-Yvette. Due to the close collaborations between the two labs this included a visit of Tefor Paris-Saclay (formerly known as Tefor Core Facility). Here is his France Inter report of this visit.
TEFOR Paris-Saclay proposes integrated services in genome editing, transgenesis, 3D image acquisition and annotation focusing on aquatic organisms. In this context TEFOR Paris-Saclay performs technological research and development and establishes programs for the communities working on these models.
As part of the TEFOR infrastructure, TEFOR Paris-Saclay is dedicated to Research and Development of new techniques in the field of fluorescence microscopy. Its development activities reach from the enhancement of the specimen preparation over testing and co-developing new microscopes and optics to data management, visualization and analysis. Excurses in the development of screening pipelines as well as other model organisms than Zebrafish broadens the scope and capabilities of this platform.
Conducting our own research projects is the science we studied for. Using the tools developed in the Tefor Infrastructure projects become possible, which wouldn't be feasible in smaller teams.
From Tissue Clearing over Deep Imaging to Big Data Management; we developed a line of tools and techniques for working on challenging problems in imaging and visualization of big specimens.