Fast 3D-screen for toxicology

Some model organisms are currently used for image-based fast toxicological screening both in industrial and academic environment. Nevertheless the analysis is usually performed at cell scale and/or in a two-dimensional context, therefore losing the opportunity to observe long-time effects on whole three-dimensional samples. The toxicity of some substances can also be deduced by behavior analysis, but this kind of screening can lack precision in quantifying the dosage of tested molecules. Fluorescence imaging combined with the dedicated CLARITY/bleaching protocols developed in our group and appropriate antibodies provides nowadays the opportunity to observe whole internal structures in animal samples in a three-dimensional context even at late stage of development. We are cooperating with a French industry for developing an image-based HCS (high content screening) strategy depending on the three-dimensional morphology of medaka fry. To this aim we are focusing on the development of an automatic workflow for the identification and reconstruction of the shapes of interest. Once settled up, the pipeline will allow to compare different animal samples thus providing an automatic tool for reliable screening of drugs in a relative short time.
Pierre Affaticati